Graduate Studies in Chemistry

Department of Chemistry University of Oxford

Room temperature high-resolution X-ray spectroscopy of electrochemically-controlled redox metalloproteins at intense beamlines

Supervisors: Prof. Kylie A. Vincent, Dr Philip A. Ash from University of Oxford, Dr. Stephen P. Best from University of Melbourne and Dr Sofia Diaz-Moreno from Diamond Light Source Ltd.

Project Description:

This project addresses key challenges in X-ray absorption spectroscopy studies of metal- containing proteins, namely how to control and protect against photoreduction and X-ray damage and how to generate protein samples in well-defined oxidation states. These questions are highly relevant on beamlines like I20, where the high intensity allows study of low protein concentrations (typical for biological studies) but in turn is more likely to induce damage to the protein. Exploiting expertise of Vincent and Ash in Oxford on electrochemical control of metalloproteins, we explore the applicability of a pulsed solution electrochemical flow approach, demonstrated by Best, to protect proteins during room temperature solution measurements of defined redox states. We will set up this approach on the I20 beamline and aim to generate data on a range of metalloprotein sites which will serve as models for understanding XAS of more complex enzymes.

This will include electron transfer proteins with haem and non-haem iron sites, copper and nickel sites and an iron-sulphur cluster. We then extend the study to a direct electrochemical approach in which the protein of interest is immobilised on a carbon electrode and exchanges electrons directly, enabling fast catalytic oxidation and reduction reactions in an enzyme to be controlled in situ during XAS measurement.
Research will be carried out 50% at Diamond Light Source which is the UK’s national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire. The remaining 50% research will be conducted in the Department of Chemistry at the University of Oxford. The Science Transit shuttle provides quick and convenient travel between the University of Oxford Science Area and Harwell Campus.

Funding Notes:

This project is funded for four years by the Biotechnology and Biological Sciences Research Council – BBSRC. BBSRC eligibility criteria apply. EU nationals who do not meet BBSRC residence criteria are encouraged to contact the Programme Administrator (lorraine.damerell@dtc.ox.ac.uk ) to check their eligibility for BBSRC funding before submitting a formal application. Successful students will receive a stipend of no less than the standard RCUK stipend rate, currently set at £14,777 per year, which will usually be supplemented by the industrial partner.

For further information about the project, please email: diamond.students@diamond.ac.uk or Prof. Kylie Vincent: kylie.vincent@chem.ox.ac.uk

Application deadline: 12.00noon (UK time) on Friday, 4th May

In order to submit a formal application, please follow the link: https://www.ox.ac.uk/admissions/graduate/courses/courses-open-for-studentships?wssl=1
and scroll down to Interdisciplinary Bioscience (BBSRC Doctoral Training Partnership)

Queries relating to the application and admissions process should be directed to: lorraine.damerell@dtc.ox.ac.uk

D.Phil. opportunity with Professor Dermot O’Hare - DOH/ISTAR/2018

 

Applications are invited for a D.Phil position in Inorganic Chemistry, for three and half years, available from October 2018 to work with Professor Dermot O’Hare in the Chemistry Research Laboratory, University of Oxford, UK. The subject of the thesis will be “Synthesis of supported permethylindenyl zirconocene catalysts for ethylene polymerisation”. This studentship is funded by SCG Chemicals Co., Ltd (Thailand).
Polyethylene is the most widely used polyolefin with a global production in 2011 of over 75 million tons per year. Innovation in both the synthesis and the properties of polyethylene is still at the forefront in both industry and academia. Using supported metallocene catalysts is an important and growing component in polyethylene production.
This studentship will involve synthesis, characterisation of new permethylindenyl organometallic complexes followed by formation of solid catalysts and their use in various polyethylene applications. The project will involve the synthesis of oxygen and moisture sensitive organometallic compounds and the study of their properties and function as effective solid catalysts.
The candidate is expected to have a strong interest in chemical synthesis, structural characterisation, to be skilled in oxygen- and moisture- sensitive organometallic compound synthesis and keen to work in collaboration with industry. Experience in solid state chemistry would be an advantage. The candidate should also have II:1 or above in their first degree or higher degree in Chemistry, or a related science discipline.

This studentship will cover College and University fees (Home/EU fees rate) plus provide a maintenance grant at £14,777 per annum for UK and EU citizens.

Closing date: 12.00noon on Friday 20th April 2018

Candidates should submit a formal application for a DPhil in Inorganic Chemistry via Oxford online application system, quoting: DOH/ISTAR/2018

For enquires relating to the application and admissions process please contact: Aga Borkowska, Graduate Studies Administrator: graduate.admissions@chem.ox.ac.uk  +44 (0) 1865 272569

For informal enquiries please contact Dr. Jean-Charles Buffet, Deputy Director SCG-Oxford Centre of Excellence in Chemistry: jean-charles.buffet@chem.ox.ac.uk

For a summary of the group’s current research interests please see: http://ohare.chem.ox.ac.uk/ 

 

D.Phil. opportunity with Professor Dermot O’Hare - DOH/LDH/2018
 

Applications are invited for a D.Phil position in Inorganic Chemistry, for three years, available from October 2018 to work with Professor Dermot O’Hare in the Chemistry Research Laboratory, University of Oxford, UK. The subject of the thesis will be “Development of new applications of layered double hydroxide”. This studentship is funded by SCG Chemicals Co., Ltd (Thailand).
   Layered double hydroxides (LDHs), also known as hydrotalcites, exhibit rich solid state chemistry since both the composition of the metal layers and the intercalated anions can be varied. They have captured much attention in recent years due to their impact across a range of applications such as catalysis, optics, medical science and in inorganic-organic nanocomposites. Recently, we have reported the synthesis of a new family of dispersible, hydrophobic LDHs exhibiting very high surface areas and pore volume, nearly two orders of magnitude higher than conventional LDHs.

   This studentship will involve synthesis, characterisation and testing of new layered double hydroxides
for a range of applications.

   The
candidate is expected to have a strong interest in chemical synthesis, solid-state chemistry and characterisation, and keen to work in collaboration with industry. Experience in catalysis would be an advantage. The candidate should also have II:1 or above in their first degree or higher degree in Chemistry, or a related science discipline.

This studentship will cover College and University fees (Home/EU fees rate) plus provide a maintenance grant at £14,777 per annum for UK and EU citizens.

Closing date: 12.00noon on Friday 20th April 2018


Candidates should submit a formal application for a DPhil in Inorganic Chemistry via Oxford online application system quoting: DOH/LDH/2018

For enquiries relating to the application and admissions process please contact: Aga Borkowska, Graduate Studies Administrator: graduate.admissions@chem.ox.ac.uk +44 (0) 1865 272569

For
informal enquiries please contact Dr. Jean-Charles Buffet, Deputy Director SCG-Oxford Centre of Excellence in Chemistry: jean-charles.buffet@chem.ox.ac.uk

For a summary of the group’s current research interests please see: http://ohare.chem.ox.ac.uk/